Part:BBa_K4643013
Lactone esterase
Responsible for the lactonization of acidic sophorolipids (SLs). Catalyzes the intramolecular esterification (ring closure) between the carboxyl group of the hydroxy fatty acid and the hydroxyl group of sophorose C6 of acidic SLs. Lactonizes acetylated acidic SLs, producing di-acetylated C18:2, C18:1 and C18:0 lactonic SL, and mono-acetylated C18:1 lactonic SL. Does not catalyze the reverse reaction, the hydrolysis of lactonic SL into acidic SL, nor hydrolysis of triglycerides or paranitrophenyl esters. Sophorolipids (SLs) are a family of fungal biosurfactants that have been commercialized as biodegradable detergents in ecological cleaning solutions. They naturally exist in two forms, a closed lactone and an open acidic form. Each form has distinct properties, acidic SLs have better foaming properties, while lactonic SLs are better in surface tension reduction and have antimicrobial activity. Lactone esterase, catalyzing the final conversion of the open acidic to the closed lactonic form, provides a biotechnological opportunity to engineer and regulate the production of SLs in S.bombicola, which naturally produces a mixture of acidic and lactonic SLs.
pH dependence : Optimum pH is 3.5-6. Stable from pH 3.5 to pH 8 Temperature dependence : Optimum temperature is 20-50 degrees Celsius.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 925
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 925
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 925
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 925
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 925
Illegal NgoMIV site found at 485 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 553
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